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Nanoparticles were diluted in phenol red-free DMEM/HAMs F-12 medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 5% FBS at a final concentration of 100 g/ml and added to the donor compartment (0.2 ml)

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doi: 10.1001/archneurol.2011.125

Certain proteins were present at much higher levels in the CFS groups with the authors suggesting that decreased plasma efflux of brain-related proteins could lead to the build-up in CFS/GWS